For example:

def formatList(seq):
    seen = set()
    seen_add = seen.add
    return [ x for x in seq if not (x in seen or seen_add(x))]

There are loads of topics about this on stackoverflow and Google. This example is taken from http://stackoverflow.com/questions/480214/how-do-you-remove-duplicates-from-a-list-in-python-whilst-preserving-order and also keeps the order of your list!

A second option could be to create a second list, which only contains the clean values. In case you would like to keep both lists.

for i in mylist:
     if i not in newlist:
          newlist.append(i)

It can be easily done by given python code:

duplicate_list = [1, 3, 5, 1, 4, 5]

new_list = list(set ( duplicate_list))

 Thanks, Husain 

Take a look at this blog